Oxymatrine Suppresses Colon Cancer by reducing microRNA-21 and microRNA-141 and Targets Epithelial-Mesenchymal Transition

Colon cancer constitutes a major challenge all over the world, which has fourth highest incidence and third mortality rate of cancers. Although therapeutic modalities have been improved in recent years, 5 y survival remains low. Oxymatrine is one main active compounds extracted from roots Sophora flavescens was found with effect for colon Recently, microRNAs recognized as type diagnostic prognostic biomarker are implicated variety biological activities underlying tumourogenesis development. This study firstly evaluated anti-tumor efficacy oxymatrine via cell viability assay, colony formation assay quantification E-cadherin/vimentin metric epithelial-mesenchymal transition. MicroRNA profile conducted to identify effective involved pharmacological mechanism oxymatrine. MicroRNA-21 microRNA-141 were most differentially expressed microRNAs, overexpression both resulted mitigation Phosphatase tensin homolog predicted be microRNA-21 microRNA-141, validated luciferase assay. Silencing phosphatase increased level phosphorylated-protein kinase B phosphorylated-nuclear factorkappa transfection antagomir yielded increase them, suggesting that microRNA-21/phosphatase homolog/protein microRNA-141/phosphatase